Table 2.
Correlation of gene expression data (fold changes) obtained by DNA-microarray and real time qPCR
|
MAP acid-nitrosative stress transcriptome | ||||||
|---|---|---|---|---|---|---|
| Gene ID | Gene name | Gene Product | Microarray fold change | P-value | Real Time-qPCR fold change | SD |
|
MAP 3738c |
- |
Methyltransferase type 12 / Cyclopropane-fatty-acyl-phospholipid synthase |
-2.78465771 |
0.00216317 |
-2.89367248 |
0.17 |
|
MAP 3522 |
oxyS |
Transcriptional regulator, oxyS |
4.02084912 |
0.00065264 |
2.66363166 |
0.60 |
|
MAP 1643 |
aceAb |
Isocitrate lyase |
7.02500864 |
0.00052984 |
4.30330061 |
0.07 |
|
MAP THP-1 infection transcriptome | ||||||
|
Gene ID |
Gene name |
Gene Product |
Microarray fold change |
P-value |
Real Time-qPCR fold change |
SD |
|
MAP 0654 |
phoT |
Phosphate transporter ATP-binding protein |
-42.44433187 |
0.02392446 |
-16.81349291 |
0.91 |
|
MAP 1407 |
- |
ADP-ribose pyrophosphatase |
69.43061281 |
0.04255943 |
27.68837536 |
0.74 |
|
MAP 1317c |
- |
Acid-resistance membrane protein |
4.39998925 |
0.00351578 |
2.90831542 |
2.42 |
|
MAP 1535 |
pgsA2 |
CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase |
6.40855813 |
0.00166329 |
2.51498937 |
6.99 |
| MAP 2055 | - | Cystathione beta-lyase | -9.04737958 | 0.00004972 | -36.48386353 | 0.64 |
Selected MAP genes were validated for their expression profile by Real-Time qPCR to corroborate similar results in microarray data. Three selected genes are shown for the MAP acid-nitrosative stress transcriptome whereas five genes are shown for MAP THP-1 infection transcriptome. Gene ID: Gene identification code; SD: Standard deviation.