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. 2012 Apr 23;9:72. doi: 10.1186/1742-2094-9-72

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Copyright ©2012 Ramesh et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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B. burgdorferiinduces elevated levels of activated caspase-3 in differentiated MO3.13 cells. Flow cytometric evaluation of activated caspase-3 in differentiated MO3.13 cells following incubation in differentiation medium (top panel) and with live B. burgdorferi (MOI, 10:1) for 48 h (bottom panel). The mean percent of MBP-positive cells showing positive staining for activated caspase-3 in cultures held in medium (0.18 ± 0.1) was elevated by 30-fold in MO3.13 cells incubated with live B. burgdorferi (5.45 ± 0.48). The figure shows the results of two independent experiments.