Figure 6.

The time course of the present experiment. The rats were trained for behavioral testing at least 7 days before any surgical operation and drug or chemical injection. After the training, the rats were prepared for the sham operation or IAN-X, and the behavioral test was conducted for 14 days after the sham operation or IAN-X. In MC (both i.p. and m.i.) or saline (both i.p. and m.i.) injected rats, the drug was injected 16 h before transection, and was administrated once in a daily for 14 days after IAN-X. After behavioral test, the rats were prepared either for a second surgery prior to recording masticatory jaw movement or for perfusion prior to immunohistochemistry. In the case of IAN-X rats, the perfusion was conducted at 3 and 14 day after IAN-X. Note that the masticatory jaw movement was recorded for at least 4 days.