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. 2012 Aug 10;7(8):e43049. doi: 10.1371/journal.pone.0043049

Figure 1. A) TGFβ induction of SDC2 gene expression is IGFBP-3-dependent. Normal fibroblasts were transfected with siRNA targeting IGFBP-3 (siBP3), then stimulated with TGFβ (10 ng/ml) for 48 hours.

Figure 1

RT-PCR was used for the detection of SDC2 in 100 ng equivalent of template. β-actin was used as control. Experiments were done in triplicate. B) TGFβ induction of SDC2 protein is IGFBP-3-dependent. Primary human lung fibroblasts were transfected with siRNA targeting IGFBP-3 or IGFBP-5 as a related protein control. Fibroblasts were stimulated with TGFβ (10 ng/ml). Cellular lysates were analyzed by immunoblotting for SDC2 protein after 72 hrs. Efficiency of silencing was assessed by detecting IGFBP-3. GAPDH was used as internal control.