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. 2012 Aug 10;7(8):e43049. doi: 10.1371/journal.pone.0043049

Figure 6. IGFBP-3 induces Mknk2a expression and activation.

Figure 6

Primary normal skin fibroblasts were infected with Ad-IGFBP3 (Ad3) or control Ad (C) at an MOI of 50 for 24 h, 48 h and 72 h or 48 h, 72 h and 96 h. A) Mknk2a gene expression was examined by RT-PCR. B) IGFBP-3 activates Mnk. Primary human lung fibroblasts were treated with 250 ng/ml recombinant human IGFBP-3 for 5–30 minutes. Phosphorylation of Mnk was assessed by immunoblotting. C) IGFBP-3 activates Mknk2a. MRC-5 cells were transfected with p-Adlox expressing human Mknk2a. Cells were stimulated with rhIGFBP-3 (250 ng/ml). Lysates were examined for Mknk2 activation at 10, 30 and 60 minutes. Total Mknk2 and GAPDH were used as loading controls.