Table 1. The primer sequences for real time RT-PCR assays.

Gene	Primer Sequences	Annealing Temperature (°C)	Size (bp)
Gli1	F: 5′-TCTGCCCCCATTGCCCAC TTG-3′	56	480
	R: 5′-TACATAGCCCCCAGCCCATAC CTC-3′
Shh	F: 5′-CGGAGCGAGGAAGGGA AAG-3′	56	262
	R: 5′-TTGGGGATAAACTGCTTGTA GGC-3′
Patched1	5′-CGGCGTTCTCAATGGGCTGGT TTT-3′	54	376
	5′-GTGGGGCTGCTGTTTCGGGT TCG-3′
GAPDH	F: 5′-ACGGATTTGGTCGTATT GGG-3′	54	208
	R: 5′-TGGAAGATGGTGATGGG ATT-3′
E-cadherin	F: 5′- CAATGCCGCCATCGCT TAC -3′	56	421
	R: 5′- CAAAATGCCATCGTTGTTC ACT -3′