Figure 4. HIF-1 over-expression increases VEGF-dependent proliferation of endothelial cells.

A: Immunoblotting showing increased HIF-1α and VEGF-A₁₆₅ levels in HDMEC containing the pcDNA3-HIF-1α expression plasmid. B: Flow cytometry analysis of BrdU incorporation showing increased proliferation of cells with the pcDNA3-HIF-1α plasmid, which is prevented by VEGF neutralizing antibodies. Non-specific IgG and pcDNA3 vector were used as negative controls. C: Quantification of flow cytometry analysis of BrdU incorporation. Data represent mean (n = 3)±SD; *P<0.01.