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. 2012 Aug 10;7(8):e42913. doi: 10.1371/journal.pone.0042913

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Except for p70S6K panel in Figure 5A and the β-actin panel in Figure S1, this is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A: Immunoblotting showing the effects of rapamycin on p70S6K phosphorylation, HIF-1α expression and VEGF-A₁₆₅ expression in normal (HDMEC) or hemangioma (EC2, EC17B, EC21A) endothelial cell lysates. B and C: Luminex analysis showing reduced expression of HIF-1α (B) and VEGF-A₁₆₅ (C) in hemangioma endothelial cells treated with rapamycin. Data represent mean (n = 3)±SD; *P<0.05 compared to vehicle.