Table 4. IlvA variants have reduced activity.

ilvA allele	Proteinvariant	Activity*	µ†(Glc)	µ†(Glc Ile)
ilvA WT	WT	0.22±0.01	0.54±0.05	0.53±0.01
ilvA3210	IlvAA142T	B.D.‡	0.62±0.01	0.60±0.03
ilvA3211	IlvAG191S	0.05±0.01	0.56±0.03	0.54±0.01

^*Threonine dehydratase (IlvA) activity measured in crude extracts from DM3480 (ridA), DM7610 (ridA ilvA3210) and DM7608 (ridA ilvA3211) and reported as ΔA_{540 nm}/min/mg protein.

^†Growth rate (in h⁻¹) (µ =  ln(X/X0)/T where X =  optical density at 650 nm and T =  time in hours during logarithmic growth) for strains DM10332 (WT), DM10331 (ilvA3210), and DM11558 (ilvA3211) determined from growth in minimal medium with glucose (Glc) and glucose with isoleucine (Glc Ile).

^‡Below Detection.