Table 1. Binding affinities of 55 chemical compounds to AlinCSP1-3 proteins.
| KD (µM) | KD (µM) | ||||||
| Ligands | AlinCSP1 | AlinCSP2 | AlinCSP3 | Ligands | AlinCSP1 | AlinCSP2 | AlinCSP3 |
| Aliphatic alcohols | Ethyl heptanoate | 43.16±2.21 | 38.29±1.57 | 35.26±2.51 | |||
| 2-Hexanol | 45.36±2.43 | u.d.1 | 37.09±1.01 | Aromatic compounds | |||
| 2-Ethyl-1-hexanol | u.d. | u.d. | u.d. | Benzaldehyde | 27.53±1.96 | 28.02±2.11 | 24.02±1.76 |
| 2-Octanol | u.d. | 39.47±2.11 | u.d. | Methyl salicylate | 8.67±1.92 | 13.32±1.71 | 11.83±1.89 |
| (Z)-3-Nonen-1-ol | 21.48±1.17 | 26.43±1.87 | 25.54±1.67 | 3,4-Dimethyl-benzaldehe | u.d. | u.d. | u.d. |
| (Z)-3-Hexen-1-ol | 12.54±2.32 | 15.15±1.79 | 16.30±1.98 | Methyl phenylacetate | 32.12±1.85 | 30.56±1.92 | 22.18±0.87 |
| 2-Undecanol | u.d. | u.d. | u.d. | 2,3-Dimethylbenzoic acid | u.d. | u.d. | u.d. |
| Tetradecanol | u.d. | 35.78±3.01 | u.d. | Ethyl phenylacetate | 27.31±2.02 | 25.26±1.98 | 24.40±1.32 |
| Aliphatic aldehydes | Heterocyclic compound | ||||||
| Valeraldehyde | 17.62±2.54 | 13.42±2.41 | 11.25±1.52 | Indole | 27.12±1.91 | 22.42±2.01 | 24.65±2.16 |
| (E)-2-Hexen-1-al | 11.32±1.07 | 13.16±2.05 | 14.52±2.59 | Aliphatic terpenoids | |||
| Nonanal | 43.65±1.11 | u.d. | u.d. | Isoborneol | 24.16±1.42 | 21.11±2.04 | 28.59±1.68 |
| Decanal | u.d. | 45.87±1.71 | 42.30±2.76 | (-)-β-Citronellol | u.d. | u.d. | 43.47±0.99 |
| Dodecanal | u.d. | u.d. | u.d. | Citral | u.d. | u.d. | u.d. |
| Saturated fatty acid | Myrcene | 5.42±0.93 | 13.04±1.72 | 10.71±1.64 | |||
| hexadecanoic acid | u.d. | u.d. | u.d. | α-Terpinene | u.d. | u.d. | 45.50±2.06 |
| Aliphatic ketones | (+)-α-Pinene | 31.71±0.98 | 30.62±0.71 | 27.19±1.02 | |||
| 2-Hexanone | 19.51±1.73 | 20.41±1.82 | 22.46±1.88 | β-Pinene | 7.07±1.41 | 11.07±1.91 | 12.37±2.09 |
| 2-Heptanone | 42.92±2.89 | u.d. | 41.23±2.56 | Linalool | u.d. | 46.21±2.35 | u.d. |
| 2-Octanone | u.d. | u.d. | 39.49±1.91 | (Z)−ocimene | 28.64±1.71 | 25.13±0.93 | 22.15±1.01 |
| 2-Nonanone | u.d. | 42.43±2.21 | u.d. | Limonene | u.d. | u.d. | u.d. |
| Aliphatic esters | β-Caryophyllene | 9.22±1.08 | u.d. | 12.35±2.42 | |||
| Ethyl butyrate | u.d. | u.d. | u.d. | α-Humulene | 9.96±0.89 | u.d. | 8.94±2.97 |
| (Z)-3-Hexenyl acetate | 7.37±2.49 | 15.82±1.68 | 12.16±1.82 | Nerolidol | 28.15±1.26 | 24.38±1.36 | 22.43±1.33 |
| Butyl acetate | 26.56±1.87 | 41.24±2.89 | 34.51±2.79 | Aliphatic alkanes | |||
| Butyl butyrate | u.d. | u.d. | u.d. | Pentane | 42.21±1.95 | 39.46±2.54 | 35.62±2.61 |
| Ethyl heptanoate | 25.32±2.54 | 45.32±2.69 | 38.28±1.67 | Octane | 42.15±1.42 | 44.36±1.36 | 45.40±1.54 |
| Hexyl butyrate | 46.84±1.85 | u.d. | u.d. | Nonane | u.d. | u.d. | u.d. |
| (E)-2-Hexenyl butyrate | u.d. | u.d. | u.d. | decane | 44.55±0.75 | 46.42±0.82 | 47.23±1.18 |
| Nonyl acetate | 23.55±1.60 | 29.16±2.19 | 46.51±3.07 | Undecane | 38.45±1.54 | 39.55±2.36 | u.d. |
| Hexyl hexanoate | 44.66±2.38 | u.d. | 43.79±1.98 | Dodecane | u.d. | u.d. | u.d. |
| Butyl acrylate | 38.54±1.69 | 28.14±2.37 | 40.17±2.88 | Tetradecane | u.d. | u.d. | 46.18±2.76 |
1
u.d. indicates that the dissociation constants were not to be calculated if the IC50>50 µM. The company, CAS number and purity of all the tested chemicals are listed in Table S1.