Figure 1. Hmox1 and foxp3 mRNA expression positively correlate in pregnancy and HO-1 protein levels determine pregnancy outcome.
A: The correlation between Hmox1 and foxp3 mRNA levels in uterus of normal pregnant specimens was analyzed. The samples used in this study were uterine, decidual and placental tissues (n = 21) obtained from day 0 until 8 of pregnancy from normal pregnant (NP) females from which mRNA expression of both of Hmox1 and foxp3 was analyzed using real time RT PCR. Correlation analysis was performed using Pearson analysis program. r2 represents co-efficient of determination value while p indicates the exact levels of significance. B: Total protein was obtained from decidual tissue of day 14 pregnant abortion-prone (AP) or NP animals and HO-1 levels were determined by Western Blot. The protein expression intensity level values (n = 18) were obtained from band intensities using Quantity 1 and were correlated to the abortion rates of the respective animals using Pearson's correlation analysis. The sample distribution was tested for normality using KS test. Correlation significances are indicated by p value. C: Hmox1 mRNA levels in testicle samples of BALB/c (n = 3) and DBA/2J males (n = 5) were determined using real time RT-PCR. D: foxp3 mRNA levels in spleen samples of BALB/c (n = 5) and DBA/2J males (n = 5) were determined using real time RT-PCR. Results are presented as median mRNA levels in dot plots. The statistical differences were analyzed using non-parametric Mann-whitney-U- test which gave a p value of 0.036 (*p<0.05).