Figure 4. Splenic dendritic cells from Hmox1^+/− and Hmox1^−/− female mice are more mature than those obtained from Hmox1^+/+ controls.

Dendritic cells (DCs) were isolated from spleen of Hmox1 ^+/+ (n = 5), Hmox1 ^+/− (n = 6) or Hmox1 ^−/− (n = 4) females, cultured with (B) or without (A) LPS addition (1 µg/ml) and analyzed for the expression of maturity markers CD11c, CD80 and MHCII. In (C), the IL-10 levels secreted by DCs from these animals were measured by ELISA. Differences among the groups were analyzed by the Mann-Whitney-U test following Kruskall-Wallis test. *:p<0.05 and **:p<0.01.