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. 2012 Aug 10;7(8):e40050. doi: 10.1371/journal.pone.0040050

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© 2012 Nawaz-ul-Rehman et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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N. benthamiana plants were inoculated with GPMLCuV (GPMLCuV^A), GPMLCuV with its cognate DNA-B (GPMLCuV^A+B), GPMLCuV with its cognate DNA-B and CLCuMuB^Mul (GPMLCuV^A+B+Mul-Beta), and GPMLCuV with its cognate DNA-B and CLCuMuB^Bur (GPMLCuV^A+B+Bur-Beta). Plants were photographed at 21 dpi. Southern hybridization for each combination is shown in panels E, F and G. In each case, approximately, 0.5 ug of DNA isolated from two different plants was loaded (lanes 1 and 2) on agarose gels and transferred to nylon membranes. Panel E was probed with the C4 gene of GPMLCuV. Panels F and G were probed with the BV1 and βC1 genes of DNA-B and CLCuMuB respectively. The replicative DNA forms are indicated as single-stranded (ss), supercoiled (sc) and open-circular (oc).