Table 2. Overview of mouse batches employed.
Batch Name | Analyses | Batch Size(n = number of mice per group) |
PTSD I | Behavior (d28–30) + WB (d2, d28, d60) | Behavioral tests: n = 16 per time point; WB: n = 6 per time point |
PTSD II (confirmatory batch) | Behavior (d28–30) + WB (d60) + IHC (d60) | WB: n = 6; IHC: n = 6 |
PTSD III | Behavior (d59–60) | n = 14 (no shock), n = 16 (shock) |
PTSD IV (fluoxetine treatment) | Behavior (d59–60) + WB (d74) | Behavioral tests: n = 16 (ctrl), n = 14 (fluoxetine); WB: n = 6 |
Abbreviations: posttraumatic stress disorder (PTSD); western blot (WB); behavioral analysis (Behavior). For detailed explanation of the PTSD stress paradigms and the course of pharmacological fluoxetine treatment see methods and results chapter.