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. 2012 Aug 10;7(8):e42371. doi: 10.1371/journal.pone.0042371

Figure 4. Vegetative small RNA libraries contain mostly 20–22 nt RNAs and mating libraries contain mostly 27 nt RNAs.

Figure 4

The length distributions of sequencing reads in the small RNA range (18–30 nt) are plotted for five of the 11 libraries (comprising one replicate from each cell type or timepoint). The histograms are for distinct reads; only one occurrence of a sequence that appears more than once in a library is counted. The five representative libraries shown can be cross-referenced to table 1; VegALXC2 - veg02_01, VegALXC9 - veg09_04, Mat24hr - mat24_06, Mat48hr - mat48_08, Mat72hr - mat72_11. Black bars represent reads that mapped to the macronuclear sequence assembly. White portions of the bars represent reads that did not map to the macronuclear assembly. The vegetative strain VegALXC2 is capable of self-mating, leading to a minor peak at 27 nt.