Table 2. Soybean genes encoding putative CKX enzymes and their properties.

Gene name	Chromosome locus	Number of exonsa	FAD_binding_4b	CK-bindingb	Familyc	Length (aa)	Identity (%)d	Glycosylation sitese	TargetPf
GmCKX01	Glyma19g31620	5	1.50E-22	2.20E-112	AtCKX1/AtCKX6	544	59.1/60.8	4	C/5
GmCKX02	Glyma03g28910	7	1.20E-21	2.70E-69	AtCKX1/AtCKX6	551	49.2/49.7	4	C/5
GmCKX03	Glyma09g07190	−6	1.10E-21	4.00E-93	AtCKX2	533	45	6	M/5
GmCKX04	Glyma09g07360	6	1.40E-18	1.30E-118	AtCKX3	536	54.8	2	S/5
GmCKX05	Glyma13g16420	7	1.80E-12	1.70E-102	AtCKX3	429	42.1	7	−/2
GmCKX06	Glyma13g16430	−5	1.10E-23	1.00E-114	AtCKX3	535	54.3	1	S/3
GmCKX07	Glyma15g18560	5	2.60E-20	5.90E-118	AtCKX3	543	53.9	2	S/5
GmCKX08	Glyma17g06220	5	4.30E-23	1.60E-116	AtCKX3	535	55.8	2	S/1
GmCKX09	Glyma17g06230	−6	1.60E-20	4.90E-102	AtCKX3/AtCKX4	528	46.8/46.8	6	−/5
GmCKX10	Glyma06g03180	5	1.40E-22	9.40E-120	AtCKX5	518	64	1	S/2
GmCKX11	Glyma04g03130	5	3.20E-22	3.30E-120	AtCKX5	458	60.8	1	−/4
GmCKX12	Glyma09g35950	6	1.50E-20	1.10E-113	AtCKX6	534	68.6	3	S/1
GmCKX13	Glyma11g20860	5	5.20E-21	2.80E-114	AtCKX6	552	64.4	6	M/4
GmCKX14	Glyma12g01390	−6	2.20E-21	6.90E-67	AtCKX6	442	55.5	1	−/4
GmCKX15	Glyma04g05840	6	3.40E-11	1.90E-101	AtCKX7	494	58.6	2	−/2
GmCKX16	Glyma14g11280	−4	7.40E-29	1.20E-103	AtCKX7	513	61.5	3	−/2
GmCKX17	Glyma17g34330	4	3.10E-26	5.90E-103	AtCKX7	513	58.6	2	−/2

^aMinus signs represent genes located on opposite strand

^bPFAM e-values for having the indicated protein domains

^cClosest homologs from Arabidopsis

^dPercentage of identical amino acids with the closest Arabidopsis homologs

^eGlycosylation sites were predicted with NetNGly (http://www.cbs.dtu.dk/services/NetNGlyc/); 1-5, highest to lowest possibility

^fLocalization predicted with TargetP (http://www.cbs.dtu.dk/services/TargetP/); M, mitochondria; C, chloroplast; S, secretory pathways; “–”, not known location; 1-5, highest to lowest possibility