Figure 1. Deletion of gaf1⁺ causes reduced growth on nitrogen-starved medium.

Cells grown to mid-log phase in EMM2 for 18 h were serially diluted 5-fold and spotted on agar plates. The plates were incubated at 30°C for 3 d. (A) Cells of WT (972) and gaf1Δ (KL230) strains were spotted on EMM, EMM-N, and EMM-G agar plates. (B) Cells of WT(ED665)/pREP42, gaf1Δ(KL210)/pREP42, WT(ED665)/pREP42-Gaf1, and gaf1Δ(KL210)/pREP-Gaf1 strains were spotted onto EMM-N plates with (+B₁) or without (−B₁) 20 µM thiamine. WT denotes the wild-type (gaf1⁺).