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. 2012 Aug 10;7(8):e42409. doi: 10.1371/journal.pone.0042409

Figure 2. Deletion of gaf1+ causes accelerated G1-arrest under nitrogen-starved conditions.

Figure 2

Cells grown to mid-log phase in EMM2 for 18 h were shifted to EMM-N medium, and their DNA contents were monitored by FACS analysis at intervals. The FACS data represent typical examples of three independent experiments. (A) Cells of heterothallic (h−) WT (972) and gaf1Δ (KL230) strains were shifted to EMM-N medium. (B) Cells of homothallic (h90) gaf1Δ(KL211)/pREP-Gaf1 strain were shifted to EMM-N medium with (+B1) or without (−B1) 20 µM thiamine. WT denotes the wild-type (gaf1+).