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. Author manuscript; available in PMC: 2013 Feb 9.
Published in final edited form as: Nature. 2012 Aug 9;488(7410):172–177. doi: 10.1038/nature11270

Figure 4. PVH neuron inhibition recapitulates feeding from AGRP neuron activation.

Figure 4

a, Cre-dependent rAAV expression of hM4D and GFP in PVH SIM1 neurons. b, Food intake before and after intraperitoneal CNO (5 mg/kg) in SIM1-hM4D mouse. c, Food intake before and after CNO (n = 19) or saline (n = 9). Red line: AGRP neuron-evoked feeding (ref. 4). d, Normalised break point for AGRP-ChR2 (n = 6) or SIM1-hM4D (n = 6) mice (2-way ANOVA/1-factor repetition, condition: F2,20 = 25.5, P <0.001; AGRP/SIM1: F1,20 = 0.171, P = 0.69; interaction: F2,20 = 0.45, P = 0.64; Holm-Sidak correction for multiple comparisons). Normalised to food deprivation (dep) break point. e, Scheme for co-photostimulation of AGRP axons and SIM1 neurons over the PVH, and (f) evoked food intake before, during, and after (1 h each) co-stimulation (paired t-test, n = 3). Values are means ± s.e.m. n.s.: not significant, *P < 0.05, **P < 0.01, ***P < 0.001.