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. Author manuscript; available in PMC: 2013 Feb 9.
Published in final edited form as: Nature. 2012 Aug 9;488(7410):172–177. doi: 10.1038/nature11270

Figure 6. Pharmacological dissection of AGRP neuron-evoked feeding.

Figure 6

a, Scheme depicting infusion of antagonists (purple) for GABAA receptors or Npy1r into the PVH followed by ARCAGRP→PVH axon photostimulation. b, Food intake during photostimulation (1 h) in ChR2-expressing mice (black, n = 5) and ChR2-negative mice (open bars, n = 5). 2-way ANOVA/1-factor repetition; ARCAGRP→PVH activation: F1,16 = 24.4, P = 0.001; antagonist: F2,16 = 13.0, P < 0.001; interaction: F2,16 = 8.6, P = 0.003. c, As in a except with somatic AGRP neuron activation using hM3D and CNO (0.3 mg/kg). d, Food intake (1 h) after intraperitoneal CNO injection in hM3D-expressing (black, n = 8) and non-transduced control mice (open bars, n = 5). 2-way ANOVA/1-factor repetition; neuron activation: F1,20 = 22.8, P < 0.001; antagonist: F2,20 = 14.5, P < 0.001; interaction: F2,20 = 0.37, P = 0.694. Holm-Sidak correction for multiple comparisons. Values are means ± s.e.m. n.s.: not significant,*P < 0.05, ***P < 0.001