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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Insect Biochem Mol Biol. 2012 May 14;42(9):610–620. doi: 10.1016/j.ibmb.2012.04.008

Figure 1. Schematic representation of the genomic region encoding the An. gambiae cE5.

Figure 1

The putative transcription start site (tss), start (ATG) and stop (TAA) codons, alternative polyadenylation signals (p1–p3) are shown. Exons are boxed and coding region is shaded. Introns and genomic flanking regions are represented as lines. Numbers represents length in base pairs. On the top the three cE5 transcripts with different 3′UTRs are represented. The distance between stop codon and polyadenylation signals is indicated (56, 368, and 450 nucleotides, respectively). At the bottom the different primer pairs used for the RT-PCR and 3′-RACE are indicated along with the size of the expected PCR fragments (see also text for additional details).