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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Insect Biochem Mol Biol. 2012 May 14;42(9):610–620. doi: 10.1016/j.ibmb.2012.04.008

Figure 3. Expression and purification of the recombinant cE5 protein.

Figure 3

SDS-PAGE analysis of protein fractions containing the recombinant cE5 after the different purification steps. M, Prosieve QuadColor Protein Marker, 4.6–300 kDa (Lonza, Cologne, GmbH). (a) Expression levels of the MBP-cE5 fusion protein after induction. I, induced; NI, non induced. (b) Purification of MBP-cE5 by Ion-Exchange Chromatography (IEC). (c) Recombinant MBP-cE5 after Affinity Chromatography (AC). (d) Final purification by TEV cleavage (TC) and RP-HPLC (LC).