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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Insect Biochem Mol Biol. 2012 May 14;42(9):610–620. doi: 10.1016/j.ibmb.2012.04.008

Figure 4. Tissue-specific expression of the cE5 protein in adult female salivary glands.

Figure 4

(a) Western blot analysis on female salivary gland extracts and recombinant cE5 protein. Rec, ~100 ng recombinant cE5; SG5, salivary extracts from 5 pairs of glands; SG1, salivary extracts from 1 pair of glands. (b) Silver staining (top) and western blot (bottom) on protein extracts from different tissues. The silver stained gel contains approximately 1/10th the protein amount as compared to western blot. The exact amount of protein extracts loaded on the gels for silver staining (s) and western analysis (w) were as follows. SG, female salivary glands: 8 pairs (w), 1 pair (s). G, midguts: 35 (w), 3.5 (s). H, heads: 35 (w), 3.5 (s). C, carcass (leftover after salivary glands, heads and midgut dissection): 11 (w), 1 (s). M, adult males: 11 (w), 1.5 (s). Numbers on the right of each panel refer to molecular weights in kDa.