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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Insect Biochem Mol Biol. 2012 May 14;42(9):610–620. doi: 10.1016/j.ibmb.2012.04.008

Figure 5. Effects of cE5 and salivary extracts from An. gambiae and Ae. albopictus on thrombin activity.

Figure 5

The activity of thrombin (10 nM) in the presence of recombinant cE5 (1 to 20 nM) or salivary extracts from An. gambiae and Ae. albopictus (0.1 to 2.0 salivary gland pairs) was measured following the hydrolysis of the chromogenic substrate Benzoyl FVR-pNA. The activity of thrombin in the absence of inhibitor or SGE was assumed as 100%. The nanomolar amounts of cE5 protein used in the assay are shown on the X axis; numbers in parentheses refer to salivary gland pair equivalents. The mean of three independent experiments (each in triplicate) are reported. Error bars represent 95% CI.