Table 1.

Specificity of cE5 to thrombin

Enzyme (nM)	% activity
Thrombin (0,03)	13.3 ± 0.6
Factor Xa (0,1)	103.8 ± 4.0
Kallikrein (0,04)	105.2 ± 2.7
Chymase (0,9)	102.7 ± 7.9
Trypsin (0,1)	101.7 ± 4.9
a-Chymotrypsin (0,075)	102.5 ± 1.4
b-Tryptase (0,01)	104.7 ± 4.3
Elastase (0,18)	90.5 ± 5.2
Cathepsin G (6,7)	95.6 ± 2.0
u-PA (0,5)	98.9 ± 5.1
Plasmin (0,4)	102.3 ± 2.7
Matriptase (0,1)	102.7 ± 8.3
Factor XIa (0,06)	100.6 ± 1.6
Factor XIIa (0,1)	106.3 ± 3.7
t-PA (0,06)	106.9 ± 2.1

cE5 (600 nM) was incubated with the amount of protease indicated in parentheses in the presence of the specific fluorogenic substrate as described in the supplementary materials and methods (Online Resource 1). Enzyme activities in the absence of inhibitor were set as 100%. Data represent the mean ± SEM (Standard Error of the Mean) of triplicate experiments. Bold denotes statistically significant difference (t-test, P<0.05).