Fig 4.

Example of 454 TP53 sequencing results in a matched (A) primary and (B) liver metastasis pair (patient 8). The 454 sequencing showed the presence of a KRAS mutation (G12D) in 42.87%, 30.04%, and 53.67% of alleles in the adenomatous component, invasive component of the primary lesion, and metastasis, respectively. A TP53 R306* mutation was not detected in the adenomatous component of the primary lesion, whereas it was present in 15.27% and 39.5% of alleles in the invasive component of the primary tumor and in the metastasis, respectively. Genotyping of frozen primary tumor (intramucosal carcinoma) and the liver metastasis revealed concordant mutations in KRAS (G12D) but discordant TP53 results (data not shown).