Table 2.

Alterations of the MAPK/ERK and PI3K/AKT pathways in histologically anaplastic PA (n = 25), clinically aggressive PA (n = 24), and conventional PA (n = 43)

Case #	Age	Sex	Location	NF1 clinical status	Tumor type	PTEN FISH	PTEN qRT-PCR FC FINAL	pAKT IHC	pS6 IHC	BRAF FISH	pERK IHC	P16 FISH
1	5	M	Cerebellum	Sporadic	APA		1.07	2	2	Dup	3	Normal
2	70	M	Cerebellum	Sporadic	APA	Het Del		2	2	Normal		HD
3	28	M	Cerebellum	Sporadic	APA	WT		2	3		2
4	11	M	R frontal lobe	Sporadic	APA	WT	−13	3	3	+7	3	Normal
5	36	F	Cerebellum	Sporadic	APA	Het Del	−19	1	3	Dup+ 7	3	Normal
6	29	M	Cerebellum	NF1	APA	Het Del	−3.9	2	3	Normal	3	HD
7	20	M	Cerebellum	Sporadic	APA	WT		1	2	Dup	2	Normal
8	27	M	Cerebellum	Sporadic	APA	Het Del	1.9	2	3	Dup	2	HD
9	75	M	L temporal lobe	Sporadic	APA	WT		2	1	+7	3	Normal
10	10	M	3rd ventricle	Sporadic	APA	WT		3	2	+7	3	Normal
11	39	F	Cerebellum	Sporadic	APA		−1.44	3		Dup		Normal
12	46	M	L parietal lobe	NF1	APA	WT	−6	2			0
13	46	F	R temporal lobe	NF1	APA	WT	−3.6	0	0	+7	3	Normal
14	73	F	Tectum	Sporadic	APA	WT	−7	2		+7	3	Normal
15	51	M	Spinal cord	Sporadic	APA	Het Del	−1.75	2	3	Normal		Normal
16	19	M	Cerebellum	NF1	APA	WT
17	26	M	Cerebellum	NF1	APA	WT
18	16	M	Spinal cord	Sporadic	APA	Het Del		2		+7		Normal
19	49	F	Cerebellum	Sporadic	APA	WT				0
20	21	F	Medulla	Sporadic	APA	WT		2		+7		Normal
21	33	M	L parietal lobe	NF1	APA	WT
22	55	F	Cerebellum	Sporadic	APA			3		+7
23	41	M	Pineal region	Sporadic	APA		−1.9
24	11	F	R occipital lobe	NF1	APA				3		3
25	14	M	Posterior fossa	Sporadic	APA				0	Failed
26	39	F	Cerebellum	Sporadic	Agg PA	WT		1	0	Normal	3
27	4	F	Suprasellar	Sporadic	Agg PA			2	0	Dup	3
28	28	M	3rd ventricle	Sporadic	Agg PA			0		Normal	2
29	32	M	R lat ventricle	Sporadic	Agg PA	WT		1	3	+7	3
30	10	M	R parietooccipital	Sporadic	Agg PA	WT		0	0	Dup
31	23	F	4th ventricle	Sporadic	Agg PA	WT		1	1	+7	3
32	4	F	R thalamus	NF1	Agg PA
33	6	F	L frontal lobe	Sporadic	Agg PA	WT		0	2	Normal	3	Normal
34	26	M	R temporoparietal	Sporadic	Agg PA	WT		2	0	+7	3	Normal
35	50	M	Cerebellum	Sporadic	Agg PA
36	39	F	L frontal lobe	Sporadic	Agg PA			0	0		3	Normal
37	1	F	Posterior fossa	Sporadic	Agg PA	WT	−1.56	2	2	Dup	3	Normal
38	42	F	L temporal lobe	Sporadic	AggPA	WT	−1.05	2	0	Dup	3	Normal
39	44	M	R frontal lobe	Sporadic	Agg PA	WT		2		Dup	3	Normal
40	6	F	Posterior fossa	Sporadic	AggPA	WT		2	3	Normal	2	Normal
41	18	M	Cerebellum	Sporadic	Agg PA	WT		1	0	Dup	2	Normal
42	37	M	Cerebellum	Sporadic	Agg PA			2		Normal	3	Normal
43	23	F	L thalamus	Sporadic	Agg PA	WT	1.04	2	1	Normal	3	Normal
44	22	M	3rd ventricle	Sporadic	Agg PA	WT	−2.3	2	3	Dup	2	Normal
45	7	M	3rd ventricle	Sporadic	Agg PA	WT		1	3	Normal	2	Normal
46	3	M	Cerebellum	Sporadic	Agg PA			0	0			Normal
47	34	M	Posterior fossa	Sporadic	Agg PA				3
48	2	M	Cerebellum	Sporadic	Agg PA				2			Normal
49	23	M	R temporal lobe	Sporadic	Agg PA		−2.6			Normal
50	7	F	L temporal lobe	Sporadic	PA			1	0	Normal	2
51	17	M	L frontal lobe	NF1	PA	WT	−1.72	2	2	Normal	3
52	14	F	3rd ventricle	Sporadic	PA	WT		2	2	Normal	2
53	13	M	R frontal lobe	NF1	PA	WT	1.64	2	2	Normal	3
54	33	M	Spinal cord	Sporadic	PA	WT			0	Failed	2
55	10	M	Cerebellum	NF1	PA		−2.56	0	3	Normal	3
56	22	M	R parietal lobe	Sporadic	PA	WT		0	0	Normal	2
57	9	F	Suprasellar	Sporadic	PA			1	0	Normal	2
58	14	F	R temporal lobe	Sporadic	PA	WT		0	0	Normal
59	18	F	Cerebellum	Sporadic	PA	WT		0	0	Normal	2
60	2	M	L optic nerve	NF1	PA		−1.78
61	21	M	L parietooccipital	Sporadic	PA	WT		1	0	Dup	2
62	5	M	Posterior fossa	Sporadic	PA	WT		1	0	Normal	2
63	21	F	Temporal lobe	NF1	PA			1	3	Normal	3
64	5	F	L optic nerve	NF1	PA			0			2
65	2	F	L lat ventricle	NF1	PA			0	0	Normal	2
66	30	F	R lat ventricle	NF1	PA			0	3	Normal	3
67	18	F	Cervicomedullary	Sporadic	PA	WT		0	2	Dup	2
68	4	M	Posterior fossa	Sporadic	PA	WT		0	0	Normal	2
69	16	M	Posterior fossa	Sporadic	PA	WT		2	3	Normal	3
70	58	M	L frontotemporal	Sporadic	PA		−2.6			+7
71	11	F	Midbrain	Sporadic	PA				0	Dup	2
72	1	F	Posterior fossa	Sporadic	PA	WT		0	0	Dup	2
73	37	M	L frontal lobe	Sporadic	PA	WT			2	+7	3
74	37	F	L thalamus	Sporadic	PA				2		3
75	13	F	Posterior fossa	Sporadic	PA	WT		1	3	Normal	3
76	5	F	Suprasellar	Sporadic	PA	WT		0	1	Dup	2
77	12	M	R thalamus	Sporadic	PA			0	1	Normal	3
78	46	F	Cerebellum	Sporadic	PA	WT		1	0	Dup	2
79	18	F	3rd ventricle	Sporadic	PA				3	Normal	3
80	47	F	Hypothalamus	Sporadic	PA			1	0	+7	3
81	4	M	Cerebellum	Sporadic	PA	WT		1	0	Dup	3
82	19	M	4th ventricle	Sporadic	PA	WT		0	0	Dup	1
83	7	M	Suprasellar	Sporadic	PA	WT		0	1	Normal	3
84	11	F	Posterior fossa	Sporadic	PA	WT		1	0	Dup	3
85	3	F	R optic nerve	NF1	PA			0	0		1
86	2	M	Suprasellar	Sporadic	PA			2		Dup	3
87	3	F	Cerebellum	Sporadic	PA	WT		0	0	Dup	2	Normal
88	13	M	R temporooccipital	NF1	PA	WT		1	3	+7	3	Normal
89	45	F	Brainstem	NF1	PA			0	0		2
90	3	M	Cerebellum	Sporadic	PA		−1.7			Normal
91	19	M	4th ventricle	Sporadic	PA		3.66			Dup
92	76	F	Cerebellum	Sporadic	PA		−2.6			Normal
93	40	M	L temporal lobe	Sporadic	Control	WT	1	0	0	Normal	0
94	52	M	L temporal lobe	Sporadic	Control	WT	6.428604	0	0	Normal	0
95	1	F	Cerebellum	Sporadic	Control	WT		0	1	Normal	0

NF1 neurofibromatosis type 1, Het del heterozygous deletions, HD homozygous deletions, WT wild type