Detection of different V3 DNA in a mixture by the HIV-1 V3 denaturing HTA. 1. Mixture of 50ng each V3 DNA from clone SW87, 91US005, WC205PX4, WC207PX4 and W212PX4. 2. 50ng DNA from V3 clone SW87. 3. 50ng DNA from V3 clone 91US005. 4. 50ng DNA from V3 clone WC205PX4. 5. 50ng DNA from V3 clone WC207PX4. 6. 50ng DNA from V3 clone W212PX4. p. probe only. Sequence alignment of V3 clone SW87, 91US005, WC205PX4, WC207PX4 and W212PX4 can be found at Fig. 3(B).