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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: J Virol Methods. 2012 Jun 21;185(1):108–117. doi: 10.1016/j.jviromet.2012.06.013

Fig. 5.

Fig. 5

Tropism determination of HIV-1 from patient plasma samples by the HIV-1 V3 denaturing HTA. (A) HIV-1 V3 denaturing HTA result using plasma sample of patient WC202. 1. 100ng of purified HIV-1 V3 RT PCR product from plasma sample of patient WC202. 2. 50ng V3 DNA from clone WC202PR5b. 3. 50ng V3 DNA from clone WC202PR5f. 4. 50ng V3 DNA from clone WC202PX4. p. probe only. (B) HIV-1 V3 denaturing HTA result using plasma sample of patient WC28. 1. 100ng of purified HIV-1 V3 RT PCR product from plasma sample of patient WC28. 2. 50ng V3 DNA from clone WC28PR5. 3. 50ng V3 DNA from clone WC28PX4a. 4. 50ng V3 DNA from clone WC28PX4c. 5. 50ng V3 DNA from clone WC28PX4b. p. probe only. (C) Amino acid sequence alignment of V3 clones from samples WC202 and WC28.