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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Psychoneuroendocrinology. 2012 Apr 10;37(10):1688–1699. doi: 10.1016/j.psyneuen.2012.02.018

Figure 6.

Figure 6

Rapidly isolated hippocampal microglia from adult male and OVX ± E2 female rats were incubated with 0, 10 or 100 ng/ml LPS for 3 h. IL-1β, MD2, CD14 and TLR4 mRNA was measured. LPS significantly increased IL-1β and CD14 mRNA in all groups. Supplemented E2 in the OVX females significantly increased IL-1β mRNA compared to all other groups at both doses of LPS. There was a significant LPS dose response on CD14 mRNA, with males and females supplemented with E2 having the greatest CD14 mRNA expression. Females and males with E2 had significantly greater TLR4 mRNA in response to LPS compared to those not supplemented with E2. Data are expressed as mean ± SEM (n=6–10 per group). *** P<0.001 compared to all other groups at the same dose of LPS.