Figure 2.

LMD is induced by visual stimuli. a. Mating duration assays of group- or singly reared CS males in constant darkness for 5 days and of GMR-Hid, blind animal. From left to right, n=29, 29, 15, and 19. t=1.247 and 0.4406. b. Mating duration assays of various vision, olfactory, and auditory mutants. The genotypes are indicated below the bars. From left to right, n=17, 20, 29, 29, 23, 27, 19, 26, 44, 25, 20, and 23. t=0.2103, 0.1664, 0.7524, 1.672, 3.436, and 4.724. c. Mating duration assays of group- (white bars), or singly reared (black) males, or males reared with 3 Drosophila simulans or Drosophila virilis (red), w⁺ strains (pink), or w1118 males (grey). The genotype of the w⁺ strain was w*;tub-GAL80^ts. Eye color of w*;tub-GAL80^ts strain is bright red. From left to right, n=22, 22, 27, 27, 30, and 24. F=20.83. d. Mating duration assays of group-reared males with a mirror or reversed mirror (G w/Mirror and G w/Mirror^rev), or singly reared males with a mirror or reversed mirror (S w/Mirror and S w/Mirror^rev) in place for 5 days. Reversed mirror is a mirror placed upside-down at the bottom of the vial, so that the male cannot see his reflection in the mirror. The color of the backside of the mirror is dark green. From left to right, n=15, 25, 24, and 23. F=4.891. e. The presentation of a mirror to a male fly. A small round mirror was placed at the bottom of the food vial to generate the male’s reflection. f. Mating duration assays of group- (white bars), or singly reared (black) males, and males reared with 3 other males (blue) or with 3 females (red) separated by transparent film. From left to right, n=28, 22, 18, and 20. F=19.19.