Fig. 4.

The influence of Gαi, adenylate cyclase and cAMP on cilia length appears distinct from that of IL-1. a 8-BR-cAMP treatment increased primary cilia length compared with untreated controls. The effect of 8-BR-cAMP was abolished by PKA antagonists PKI and H89 such that there were no significant differences with the untreated controls. b G-protein inhibition with pertussis toxin (PT) increased cilia length in the absence of IL-1β (+++p < 0.0001). There was no significant difference between IL-1β- and PT-treated and IL-1 alone. c Adenylate cyclase inhibitor 2′-5′deoxyadenosine (DOA) increased cilia length in both control and IL-1-treated samples relative to corresponding controls (+++p < 0.0001). d IL-1 and 8-BR-cAMP combined further increased cilia length beyond that of IL-1β alone