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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Biol Blood Marrow Transplant. 2012 Jun 12;18(8):1182–1190.e1. doi: 10.1016/j.bbmt.2012.06.002

Fig. 1. Toxicity of LBH589 with QD treatment.

Fig. 1

B/c mice (n = 4-5) were lethally irradiated one day before BMT. In the day of transplantation 5 × 106 TCD B6 BM cells were transferred to B/c recipients via tail vein. Recipients were treated with PBS vehicle or different dose of LBH589 daily from day 0 to 4 via i.p injection. (A) Mice survival. (B) On day 28, recipients (n=5) treated with vehicle control or LBH589 at 2.5mg/kg BW were sacrificed, and the percentages of donor B cells (B220) T cells (CD4/CD8), DC (CD11c) and neutrophils (CD11b/Gr-1) in spleen were analyzed by flow cytometry. (C) 4×105 splenic cells from recipient mice (n=5) were stimulated with 1μg/ml anti-CD3 or 5μg/ml LPS for 3 days. The proliferation of T cells or B cells was compared by [3H]-Thymidine incorporation. CPM: count per minute. This experiment was repeated 3 times and similar phenomenon was observed. The representative data from one experiment was shown. n.s, no significant difference. *p<0.05.