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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Biol Blood Marrow Transplant. 2012 Jun 12;18(8):1182–1190.e1. doi: 10.1016/j.bbmt.2012.06.002

Fig. 7. Effect of LBH589 and SAHA on donor T cell expansion and migration in vivo.

Fig. 7

B/c mice (n = 4) were lethally irradiated and transplanted with 5 × 106 T cell depleted B6 BM cells with or without 1 × 106 T cells. Recipients were treated with PBS vehicle, SAHA (25mg/kg BW, daily treatment from day 0 to 7) or LBH589 (1.25mg/kg BW, QOD from day 0 to 12) via i.p injection. Mice were sacrificed on day 13. Splenic T cell number (A), CXCR3 expression on splenic donor T cells (B), and liver T cell infiltration (C) were analyzed by flow cytometry. The data shown are pooled from 2 replicate experiments (n = 8). (D) The flow cytometry data of H-2kb+CD4-CD8- cells in the spleen of each group. The data of one mouse from each group was shown here. Only the cells in live gate were studied. (E) The average percentage of H-2kb+ cells in spleen. Only the cells in live gate were studied. The data shown was pooled from 2 replicate experiments (n = 8). n.s, no significant difference; *p<0.05; **p<0.01; ***p<0.001.