Fig. 2.

Co-culture of mESC-OPCs with mESC-Neu. (A) Representative images showing that after 7-day co-culture with mESC-Neu, the GFP⁺ mESC-OPCs mature into MBP⁺ process-bearing oligodendrocytes. GFP in green, MBP in red and Tuj1 in white. (B) Representative images showing that after 7-day co-culture, many GFP⁺ mESC-OPCs are still at NG2⁺ OPC stage and some of them generate GFAP⁺ astrocytes. Arrows indicate the GFAP⁺ astrocytes generated by GFP⁺ mESC-OPCs. GFP in green, NG2 and GFAP in red. Blue, DAPI-stained nuclei. Scale bars represent 50 µm.