Co-culture of mESC-OPCs with mESC-Neu. (A) Representative images showing that after 7-day co-culture with mESC-Neu, the GFP+ mESC-OPCs mature into MBP+ process-bearing oligodendrocytes. GFP in green, MBP in red and Tuj1 in white. (B) Representative images showing that after 7-day co-culture, many GFP+ mESC-OPCs are still at NG2+ OPC stage and some of them generate GFAP+ astrocytes. Arrows indicate the GFAP+ astrocytes generated by GFP+ mESC-OPCs. GFP in green, NG2 and GFAP in red. Blue, DAPI-stained nuclei. Scale bars represent 50 µm.