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. Author manuscript; available in PMC: 2012 Aug 12.
Published in final edited form as: Cell. 2011 Feb 25;144(5):732–744. doi: 10.1016/j.cell.2011.02.012

Figure 5. HP1a or H3K9 HMTase Depletion Results in Formation of Rad51 Foci in Heterochromatin.

Figure 5

(A) IF analysis 60 min after IR and quantitation shows that Su(var)3–9, Su(var)3–9 + SetDB1, or HP1a depletion (quantitation is in B) results in increased Rad51 foci in DAPI-bright (dashed blue circles) and not in DAPI-weak (3- to 4-fold increase in DAPI-bright after Su(var)3–9 and SetDB1 RNAi [right]; p < 0.001; n > 100).

(B) Quantitation of γH2Av and Rad51 foci at different time points after IR shows that depletion of HP1a results in increased foci in DAPI-bright (*p < 0.01; n > 100) and not in DAPI-weak.

Graphs show mean ± SEM. Scale bars, 1 μm. See also Figure S7.