Table 1.

Binding Characteristics of IgM Natural Autoantibodies

Antibody	Antigen(s)	Method	KD (M)	kon (M−1 s−1)(b)	koff (s−1)(b)	Ref
O1(a)	2 % GalC in SLB	SPR	(2.37±0.56)×10−9	(6.98±4.92)×105	(1.10±0.51)×10−3	This study
O4(a)	2 % Sulf in SLB	SPR	(2.19±0.61)×10−9	(2.65±1.71)×105	(3.73±1.15)×10−4	This study
F5-2	Actin Myosin Tubulin	SPR	4.03 × 10−8 2.74 × 10−8 6.80 × 10−8	1.85 × 104 2.84 × 104 1.67 × 104	7.46 × 10−4 7.78 × 10−4 1.14 × 10−3	49
2E4	ssDNA β-galactosidase Insulin	ELISA	4 × 10−8 7 × 10−7 1 × 10−5	N.D.	N.D.	50
274.RA.F11	IgG Fc frag.	ELISA	4 × 10−5	N.D.	N.D.	51
274.RA.F5	IgG Fc frag.	ELISA	3 × 10−7	N.D.	N.D.	51
Ab 50	IgG Fc frag. ssDNA Insulin Tetanus toxoid	ELISA	1 × 10−5 5.6 × 10−7 1 × 10−4 6.4 × 10−6	N.D.	N.D.	52
Ab 52	IgG Fc frag. ssDNA Insulin Tetanus toxoid	ELISA	3 × 10−5 1 × 10−6 1 × 10−4 2.5 × 10−6	N.D.	N.D.	52

^(a)K_D, k_on and k_off values reported as mean ± s.e.m.

^(b)N.D. = not determined.