Table 2.
Tryptic glycopeptides identified in the bovine serum albumin protein by LC/MS/MS analysis of the lactose-BSA glycoconjugates
| Peptide |
Lactose-BSA 5.1:1 |
Lactose-BSA 19.0:1 |
||||
|---|---|---|---|---|---|---|
| Sequence (star = glycation site) | Calculated m/z (charge) | Missed cleavage | Observed m/z (charge) | Deviation (Da) | Observed m/z (charge) | Deviation (Da) |
| SLGK*VGTR (Lys 455) | 697.3568 (+2) | 1 | 697.3748 (+2) | 0.0181 | 697.3567 (+2) | −0.0001 |
| LSQK*FPK (Lys 245) | 712.3640 (+2) | 2 | 712.3812 (+2) | 0.0172 | 712.3673 (+2) | 0.0033 |
| EK*VLTSSAR (Lys 211) | 783.8912 (+2) | 1 | 783.8877 (+2) | −0.0035 | 783.8900 (+2) | −0.0012 |
| ALK*AWSVAR (Lys 235) | 789.4068 (+2) | 1 | 789.4228 (+2) | 0.0161 | 789.4125 (+2) | 0.0058 |
| LAK*EYEATLEECCAK (Lys 374) | 797.6874 (+3) | 2 | 797.7141 (+3) | 0.0267 | 797.6954 (+3) | 0.0080 |
| CASIQK*FGER (Lys 228) | 886.4066 (+2) | 1 | 886.4299 (+2) | 0.0233 | 886.4033 (+2) | −0.0033 |
| CCTK*PESER (Lys 463) | 871.8587 (+2) | 1 | 871.8738 (+2) | 0.0151 | 871.8000 (+2) | −0.0587 |
| VTK*CCTESLVNR (Lys 498) | 1021.9667 (+2) | 1 | 1021.9941 (+2) | 0.0274 | 1021.9741 (+2) | 0.0074 |
| K*FWGK (Lys 156) | 621.3007 (+2) | 1 | – | – | – | – |
| TPVSEK*VTK (Lys 495) | 782.8959 (+2) | 2 | – | – | 782.8995 (+2) | 0.0036 |
| K*VPQVSTPTLVEVSR (Lys 437) | 1108.5811 (+2) | 0 | – | – | 1108.5854 (+2) | 0.0043 |
| SLHTLFGDELCK*VASLR (Lys 100) | 841.4162 (+3) | 1 | – | – | 841.4000 (+3) | −0.0162 |