Table 3.
Glycopeptides identified in the bovine serum albumin protein by LC/MS/MS analysis of the GluC V8 digests of the lactose-BSA glycoconjugates
| Peptide |
Lactose-BSA 5.1:1 |
Lactose-BSA 19.0:1 |
||||
|---|---|---|---|---|---|---|
| Sequence (star = glycation site) | Calculated m/z (charge) | Missed cleavage | Observed m/z (charge) | Deviation (Da) | Observed m/z (charge) | Deviation (Da) |
| K*TPVSE (Lys 489) | 618.7904 (+2) | 0 | 618.7989 (+2) | 0.0085 | 618.7940 (+2) | 0.0036 |
| HVK*LVNE (Lys 65) | 707.8513 (+2) | 0 | 707.8591 (+2) | 0.0078 | 707.8565 (+2) | 0.0052 |
| K*LFTFHAD (Lys 528) | 777.8644 (+2) | 0 | 777.9566 (+2) | 0.0922 | 777.9564 (+2) | 0.0920 |
| LCK*VASLRE (Lys 100) | 826.4087 (+2) | 0 | 826.4216 (+2) | 0.0129 | 826.4137 (+2) | 0.0050 |
| VSRSLGK*VGTRCCTKPE (Lys 455) | 837.7407 (+3) | 0 | 837.7418 (+3) | 0.0011 | 837.7468 (+3) | 0.0061 |
| VSRSLGK*VGTRCCTKPESE (Lys 455) | 909.7656 (+3) | 1 | 909.7741 (+3) | 0.0085 | 909.7714 (+3) | 0.0058 |
| DKGACLLPK*IE (Lys 204) | 910.4480 (+2) | 1 | 910.4582 (+2) | 0.0103 | 910.4539 (+2) | 0.0059 |
| YAVSVLLRLAK*E (Lys 374) | 969.5198 (+2) | 0 | 969.5187 (+2) | −0.0011 | 969.4790 (+2) | −0.0408 |
| LLYYANK*YNGVFQE (Lys 183) | 1149.5389 (+2) | 0 | 1149.5413 (+2) | 0.0024 | 1149.5565 (+2) | 0.0176 |
| K*LFTFHADICTLPDTE (Lys 528) | 1242.5726 (+2) | 2 | 1242.5946 (+2) | 0.0220 | 1242.5876 (+2) | 0.0150 |
| K*SHCIAE (Lys 309) | 710.8113 (+2) | 0 | – | – | 710.8039 (+2) | −0.0074 |
| K*SLHTLFGD (Lys 88) | 797.3804 (+2) | 0 | – | – | 797.3361 (+2) | −0.0443 |
| CCDK*PLLE (Lys 304) | 805.8445 (+2) | 1 | – | – | 805.8449 (+2) | 0.0004 |
| FAK*TCVADE (Lys 75) | 808.8481 (+2) | 1 | – | – | 808.8551 (+2) | 0.0070 |
| GPK*LVVSTQTALA (Lys 597) | 930.9883 (+2) | 0 | – | – | 931.0042 (+2) | 0.0159 |
| IAHRFK*DLGEE (Lys 36) | 945.9523 (+2) | 2 | – | – | 945.9632 (+2) | 0.0109 |
| AK*DAFLGSFLYE (Lys 346) | 968.9514 (+2) | 1 | – | – | 968.9538 (+2) | 0.0024 |
| VSRSLGK*VGTRCCTK*PE (Lys 455, Lys 463) | 1029.8153 (+3) | 0 | – | – | 1029.8239 (+3) | 0.0086 |
| K*DAIPE (Lys 318) | 624.7904 (+2) | 1 | – | – | 624.8000 (+2) | 0.0096 |
| VTK*LVTD (Lys 256) | 676.3402 (+2) | 0 | – | – | 676.3457 (+2) | 0.0055 |
| K*SHCIAEVE (Lys 309) | 824.8668 (+2) | 1 | – | – | 824.8706 (+2) | 0.0038 |
| LTK*VHK*E (Lys 263, Lys 266) | 1003.9750 (+2) | 0 | – | – | 1003.9831 (+2) | 0.0081 |
| K*VTK*CCTE (Lys 495, Lys 498) | 1089.4619 (+2) | 0 | – | – | 1089.4600 (+2) | −0.0019 |
| VSRSLGK*VGTRCCTK*PESE (Lys 455, Lys 463) | 1101.8402 (+3) | 1 | – | – | 1101.8476 (+3) | 0.0074 |
| DK*GACLLPK*IE (Lys 197, Lys 204) | 1198.5599 (+2) | 1 | – | – | 1198.5604 (+2) | 0.0005 |
| KK*FWGK*YLYE (Lys 156, 160) | 1257.5868 (+2) | 0 | – | – | 1257.5983 (+2) | 0.0115 |