Figure 5. In vivo animal studies.
(a) Reversal of hyperglycemia by transplanted Pdx1- or Pdx1-VP16-expressing WB cells. Single-cell-derived Pdx1-, Pdx1-VP16-expressing WB cells or WB-GFP-expressing control cells (2 × 106 cells/mouse) were transplanted (upward arrow, Tx) into Stz-induced diabetic mice after blood glucose reached around 400 mg/dl (n = 3 mice per group). The blood glucose levels were monitored by tapping the tail vein every three days. Left nephrectomy was performed on one mouse from each group (downward arrows, indicated by Ex), and blood glucose levels were measured 24 h after removal of left kidney. The animals were sacrificed at indicated times. Mean±s.e.m., ***P<0.001. (b) Intraperitoneal glucose tolerance test (IPGTT). Mice received a loading dose of glucose (2 mg/g body weight) and the blood glucose levels were monitored at indicated times. The results represent the means±s.e.m. of the blood glucose levels of three mice in each group. Naïve mice were used as control mice for IPGTT. Mean±s.e.m., n = 3 mice per group.