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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: J Am Chem Soc. 2012 Jul 19;134(30):12798–12806. doi: 10.1021/ja304809s

Figure 3.

Figure 3

Immunofluorescence and differential interference (DI) contrast microscopy of P. pastoris overexpressing Ubiquitin. A. 24 h post-methanol induction without nitrogen starvation. B. 48 h post-methanol induction without nitrogen starvation. C. 48 h post-mixed dextrose/methanol induction. Immunofluoresent staining reveals a distribution of Ubiquitin throughout the cytosol and in protein storage bodies. Vacuoles are evident as dark regions in DI contrast images.