TABLE 4.
Changes in Mitochondrial Inner-Membrane Polarization in Hematopoietic Progenitor (32D cl 3) Cells after Irradiation (10 Gy)
| Control | + l-arginine | 10 Gy | 10 Gy + l-arginine | |
|---|---|---|---|---|
| Time = 30 min | ||||
| Green JC-1a | 1.0 ± 0.1 | 0.9 ± 0.1 | 1.1 ± 0.1 | 1.1 ± 0.1 |
| Red JC-1a | 1.0 ± 0.1 | 1.0 ± 0.2 | 0.5 ± 0.1 | 0.7 ± 0.1 |
| Ratio (red/green) | 1.0 ± 0.2 | 1.1 ± 0.2 | 0.5 ± 0.2 | 0.6 ± 0.2 |
| Time = 1 h | ||||
| Green JC-1a | 1.0 ± 0.1 | 0.9 ± 0.1 | 1.2 ± 0.1 | 1.2 ± 0.1 |
| Red JC-1a | 1.0 ± 0.1 | 1.1 ± 0.1 | 0.9 ± 0.1 | 1.0 ± 0.1 |
| Ratio (red/green) | 1.0 ± 0.2 | 1.2 ± 0.2 | 0.8 ± 0.2 | 0.8 ± 0.2 |
| Time = ± h | ||||
| Green JC-1a | 1.0 ± 0.1 | 0.9 ± 0.1 | 1.2 ± 0.1 | 1.2 ± 0.1 |
| Red JC-1a | 1.0 ± 0.1 | 1.1 ± 0.1 | 0.8 ± 0.1 | 0.8 ± 0.1 |
| Ratio (red/green) | 1.0 ± 0.2 | 1.2 ± 0.2 | 0.7 ± 0.2 | 0.7 ± 0.2 |
| Time = 18 h | ||||
| Green JC-1a | 1.0 ± 0.1 | 1.2 ± 0.1 | 1.8 ± 0.1 | 2.1 ± 0.1 |
| Red JC-1a | 1.0 ± 0.1 | 1.2 ± 0.1 | 0.7 ± 0.1 | 0.9 ± 0.1 |
| Ratio (red/green) | 1.0 ± 0.2 | 1.0 ± 0.1 | 0.4 ± 0.1 | 0.4 ± 0.1 |
Note. The uncertainties given are standard errors.
a
Data were expressed per mg protein and then normalized to the control values.