Systemic artemin administration increases CTB labeling in cuneate nucleus at 6 months. Immunofluorescence in cuneate nucleus of rats with DRC, but without artemin treatment (DRC/Vehicle), showed little CTB labeling either 2 weeks (top row) or 6 months (middle row) after DRC. After artemin administration, there was a clear increase in CTB labeling in the cuneate nucleus (DRC/artemin) at 6 months, but not at 2 weeks, after DRC. The bottom row shows high-magnification views of fluorescence for CTB in the cuneate nucleus. Scale bar represents 100 µm.