Figure 5. Dynasore prevents D₁ receptor-mediated enhancement of action potential firing in lateral dorsal striatal neurons in brain slice.

(A) Example traces showing increased firing with the D₁ receptor agonist SKF81297 (10μM) after pre-exposure to vehicle (0.2% DMSO), but not 80μM dynasore. Slices were incubated as indicated (bar in panel B) with either vehicle (0.2% DMSO, top pair of traces) or 80 μM dynasore (bottom pair of traces) prior to delivery of SKF81297. (B) Grouped data showing significant increase in firing with SKF81297 after pre-exposure to vehicle (34.7 +/− 12.8%, n=5) but not dynasore (−2.78 +/− 4.9%, n=5). Bars indicate the time course of drug perfusions relative to changes in AP firing. The percent change in number of action potentials (APs) generated relative to baseline was determined at the current step at baseline with 4 APs, or 5 APs. If no current steps at baseline had 4 APs, the baseline number of APs was determined by averaging each min for the 4 minutes before addition of DMSO or dynasore (*p<0.05 by 2-tailed unpaired t-test). See also Figure S5.