Figure A4.

Lqo-mediated growth on L-lactate under anaerobiosis requires an electron acceptor. (A) Representative growth curve of WT S. aureus strain Newman and isogenic mutants cultured anaerobically in chemically defined medium with 1.0% L-lactate as the primary carbon source. When indicated, nitrate was added (${\text{NO}}_3^{-}$ at 40 mM) as an anaerobic respiratory electron acceptor. (B) Representative growth curve of WT S. aureus strain Newman and isogenic mutants cultured anaerobically in chemically defined medium with a combination of 1.0% tryptone and l-lactate as the primary carbon source. When indicated, nitrate was added (${\text{NO}}_3^{-}$ at 40 mM) as an anaerobic respiratory electron acceptor.