Table A2.
Primers/plasmids used in this study.
| ddh-5′0.1A | 5′-AAATGCGACCATCACTGATAAACC-3′ |
| ddh-5′0.1B | 5′-TAGGGCGATAGAAACAGAATACTC-3′ |
| ddh-3′0.1A | 5′-ACGTGGTGCAGTCATCAATACACC-3′ |
| ddh-3′0.1B | 5′-ACCAACAATGTGATTGATCTGACC-3′ |
| mqo2-5′0.1A | 5′-TACTTGCTGGGGATCCAACTCACATAATGCTGCATGACCC-3′ |
| mqo2-5′0.1B | 5′-CGACTCTAGAGGATCCAAGCATTCGTTATTTTACAGGACC-3′ |
| mqo2-3′0.1A | 5′-CAGTGCAGCGGAATTCGGTAACTTCACCTTCATTCTCACC-3′ |
| mqo2-3′0.1B | 5′-TACCGAGTCGAATTCAGAAGTATTAGAACGTAACTTCCC-3′ |
| mqo1-5′0.1A | 5′-GGGGAATTCCTGTATTTGGACTTTCATCTTGTC-3′ |
| mqo1-5′0.1B | 5′-GGGGAATTCACATTCTGCTTATATTGAATTCCC-3′ |
| mqo1-3′0.1A | 5′-GGGGATCCACCAAAGATTAAAGAAATGGTGCC-3′ |
| mqo1-3′0.1B | 5′-GGGGATCCCTATTACACTGCCAAATAATTCAC-3′ |
| nar.1A | 5′-AATTTAATGGGAATTGGTCGATCC-3′ |
| nar.1B | 5′-TCCTTTCACCTCTTATGCTTACAC-3′ |
| cyd-5′0.1A | 5′-TGGTAAGTTAAATATCATGTTCCC-3′ |
| cyd-5′0.1B | 5′-AACCGACTGATTTCAACTGTATCC-3′ |
| cyd-3′0.1A | 5′-ATGGAATTAGTAAATTGCCATACC-3′ |
| cyd-3′0.1B | 5′-AAGTTTTAATTAAACTCACCACCC-3′ |
| qox-5′0.2A | 5′-CGACTCTAGAGGATCCACTCAGAAATGACTTATCATTCG-3′ |
| qox-5′0.2B | 5′-TAAGGATCGGGGATCCATGGTGCATCTTACCAGATTCG-3′ |
| qox-3′0.2A | 5′-CTGATGAATTCACATATTGGGTTATGCAAGGC-3′ |
| qox-3′0.3B | 5′-CTGATGAATTCTTAGATCAAGCAGTTAAAGCG-3′ |
| mqo2_RT.1A | 5′-TGGAGCCGGTGTACTTAGCACAAC-3′ |
| mqo2_RT.1B | 5′-AACTCACATAATGCTGCATGACCC-3′ |
| Mqo1_His.1A | 5′-TTTACCATGGCTATGACAACACAACATAGCAAAACAG-3′ |
| Mqo1_His.1B | 5′-TTTAGGATCCTTTAACTTGTAAATACTTAGTTACTTCTTC-3′ |
| Mqo2_His.1A | 5′-TTTACCATGGCTAAGTCTAATAGTAAAGACATC-3′ |
| Mqo2_His.1B | 5′-TTTAGGATCCGTTTTCGTAGTAACCTAATTCTAAGTC-3′ |
| pBT2ts | Temperature sensitive E. coli/S. aureus shuttle vector (Bruckner, 1997) |
| pBTE | 1.2 kb ermB (ErythromycinR) locus cloned into SmaI of pBT2ts |
| pBTK | 1.4 kb aph-A3 (KanamycinR) locus cloned into SmaI of pBT2ts |
| pBTS | 1.3 kb aad9 (SpectinomycinR) locus cloned into SmaI of pBT2ts |
| pTR086 | 5′ and 3′ homology regions of cydAB cloned into pBTK to yield ΔcydAB::SpR |
| pTR108 | 5′ and 3′ homology regions of mqo cloned into pBTS to yield Δmqo::SpR |
| pTR120 | 5′ and 3′ homology regions of ddh cloned into pBTS to yield Δddh::SpR |
| pDK004 | 5′ and 3′ homology regions of lqo cloned into pBTS to yield Δlqo::SpR |
| pNV004 | 5′ and 3′ homology regions of qoxBACD cloned into pBTS to yield ΔqoxBACD::SpR |
| pJS006 | 1.4 kb SpR cassette replacing the 3.5 kb ClaI fragment within the 6.5 kb narGHIJ locus cloned into pBT2ts. |
| pJF132 | C-term His6-tagged Mqo in pQE-60 |
| pJF133 | C-term His6-tagged Lqo in pQE-60 |