Figure 2.
Ab responses, time schedule for immunization and screening of the fused hybridomas. (A) The vaccine constructs elicited mCherry-specific Abs. Targeted (mMIP-1α) or non-targeted (mMIP-1αC11S) vaccine constructs, or phosphate-buffered saline, were injected into the quadriceps of mice before electroporation. Serum samples were harvested at different time points after injection and analysed by an ELISA using mCherry as coat (n = 3 mice/group, error bars indicate SD). (B) BALB/c were given DNA encoding [MIP-1α-mCherry]2 i.m. immediately followed by electroporation. Mice with the highest level of anti-mCherry Abs were re-immunized (50 μg DNA in 50 μl NaCl per quadriceps), and given an i.p. protein boost before the spleens were harvested and fused with OURI cells to generate hybridomas. (C) Hybridomas were generated, and the cell culture supernatants were tested in different dilutions for their production of anti-mCherry Abs by an ELISA containing mCherry as coat.