Table 3.
List of DNA oligonucleotides used in this study for multiplex PCR amplification of biotinylated fragments.
| Target genea | Primers for PCR |
Amplicon (bp) | Accession number | Reference | ||
|---|---|---|---|---|---|---|
| Forward sequence (5′ → 3′) | Reverse sequence (5′ → 3′) | Amountb | ||||
| Group 1 | ||||||
| wzyO26 | GGTTTATGGATTATTGCATTC | CCAATTATGACTTCATGGGT | 1.0 | 221 | AF529080 | This study |
| wzyO45 | CGTCTGGATGAAATTATGCC | GTCTGAAGACCAGCATTTCCC | 0.5 | 289 | AY771223 | This study |
| wzyO103 | ATACAAATGGCGTGGATTGG | GCCAGTAATTGACGTAACTG | 0.5 | 280 | AY532664 | This study |
| wzyO111 | GGTGTGATAGGAGCATTGGT | CAACTCCAACTGTAAGCCCG | 0.5 | 192 | AF078736 | This study |
| wzyO121 | CCCGATATTCTAGTAGCCGT | CTTCAATGAGTGCAGGCAAA | 1.0 | 235 | AY208937 | This study |
| wzyO145 | GCCCTTTTCAGTGGTGCG | GCAGCCCAATATGAAACCAT | 0.5 | 206 | AY647260 | This study |
| GROUP 2 | ||||||
| wzyO104 | GTTCATTAGATCGAGGTT | CTTGTCTGATACGGTCAAGTG | 0.4 | 162 | AF361371 | This study |
| wzxO91 | CAAGCAGGTATTTGGGATGG | TCCCCCATAGATACGAATGA | 1.0 | 248 | AY035396 | This study |
| wzyO113 | TGAGCGTTTCTGACATATGGA | GTTTCGCTGGCATATTACTG | 0.4 | 296 | AF172324 | This study |
| wzyO128 | TGGATTTGATTGGGGGAAT | TCTTGCAAAACACCGCATAC | 1.0 | 190 | AY217096 | This study |
| wzyO157 | CCGACACCAGAGTTAGAAAAG | CAGTTCTGCTCCATACGTAG | 0.4 | 156 | AF061251 | This study |
| gadB | CACGTTTTGGTGCGAAGTCT | TTGTGGACATTTTCGTCGTC | 0.2 | 175 | M84025.1 | This study |
| GROUP 3 | ||||||
| ent | CACATCATTAGAAGTTCATT | AGTCCTGCTCCCATAGCAAA | 0.5 | 342 | AAG58110 | This study |
| espP | GCACTGTCTGGCGGTGAATA | CGTCCAGATTCCCGTTTATG | 0.1 | 202 | X97542 | This study |
| katP | GCGGAAGAGAAGATGACTGG | GCACCATGTGCTTTACCAAA | 1.0 | 277 | X89017 | This study |
| nleA | TATGGTGTCCAGCTAAACAT | CCAGTCGATGCAATAGTCGA | 0.1 | 185 | BAF96541 | This study |
| saa | CCAATCAACAGTTTCGTCAA | GCAATAGCCTGTTCATCACG | 0.1 | 166 | AF325200 | This study |
| subA | CGGCTTATCATCCTGTCAGC | TATAGCTGTTGCTTCTGACG | 0.1 | 233 | AF399919 | This study |
| GROUP 4 | ||||||
| eae | CGTTACATTGACTCCCGCTT | CTCATGCGGAAATAGCCGTT | 0.2 | 188 | M58154 | This study |
| ehxA | GAGTTCTGTATCTGCGGGAG | GCAAGTTCACCGATCTTCTC | 0.75 | 339 | EF204929 | This study |
| fliCH7 | CAACAAAGCTGCAACGGTAA | GCCGCCAACTGTGACTTTAT | 0.2 | 154 | AF169323 | This study |
| per | GGTGAAGGTGGAATGGTTGTC | TCAGCAATTTCACGTTTTCGTG | 0.2 | 206 | AAG57096 | Quiñones et al. (2011) |
| stx1 | GGATGCAGATAAATCGCCAT | GAGTCTTGTCCATGATAGTC | 0.4 | 274 | AAG57228 | This study |
| stx2 | GATTTYDCACATATWTCAKTGCC | AACTCCATTAAMKCCAGATATGA | 0.75 | 155 | AAG55587 | This study |
aGene targets examined in each multiplex group.
bFinal micromolar concentration of primer used for each multiplex group in a 50-μl PCR reaction.