Table 1.
Characterization of B. ceti isolated in the Central East Pacific of Costa Rica and comparison with marine type strains.
| Species | RTD phage lysisa |
Sera againstb |
Growth on dyes (μg/ml)c |
||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Tb | Wb | Iz | R/C | CO2d | H2Se 72 h | Urease minutes | Nitrate reduction | A | M | Thionin |
Basic fuchsine | O-safranin | Omp2b restriction pattern | MLVA-16 type | ST type | ||||
| 10 | 20 | 40 | 100 | 20 | 100 | ||||||||||||||
| B. abortus biovar 1. Re | + | + | + | − | − | + | >120 | − | + | − | −(−) | −(−) | −(−) | −(−) | +(+) | +(+) | Biovar 1 type | Biovar 1 type | ST1 |
| B. ceti dolphinf | − | + | + | − | − | − | 60–90 | + | + | + | +(+) | +(±) | +(−) | +(+) | +(−) | +(−) | N(K)g | A1, A2, P | ST26g |
| B. ceti porpoisef | − | + | − | − | − | − | 60–90 | + | + | − | +(+) | +(+) | +(+) | +(−) | +(−) | +(−) | M(J) | B | ST23 |
| B. ceti humanh | − | − | − | − | − | − | 60–90 | ND | − | + | + | + | + | − | + | + | Q(I) | H | ST27 |
| B. pinnipedialis sealf | − | − | + | − | + | − | 60–90 | + | + | − | +(−) | +(−) | +(−) | +(−) | +(−) | +(−) | P(I), L(I), O(I) | C1, C2, C3 | ST25, ST24 |
aRTD, routine test dilution of phages; Tb, Tbilisi; Wb, Weybridge; Iz, Izatnagar and rough type Wb derivative (R/C).
bSerum against lipopolysaccharide epitopes, measured as agglutination with monospecific serum.
cDye concentrations expressed in μg/ml of culture medium with 10% CO2 or, within parenthesis, raw incubation without CO2.
dRequirement.
eProduction.
fReference strains (Muñoz et al., 2006; Foster et al., 2007; Hernández-Mora et al., 2008; Zygmunt et al., 2010; Guzmán-Verri et al., unpublished results).
gCharacterized only in B. ceti isolated in dolphins from the Atlantic Ocean.
hReference human isolate (McDonald et al., 2006).
ND, not done.