Figure 1.

Verification of post-transformation bacterial DNA in the ΔnudA allele PCR products on agarose gel. Two pairs of primers were designed for verification of post-transformation of bacterial transformed with ΔnudA allele PCR products. One pair was specific for nudA and the other pair was specific for the CamR gene. The wild type strain J99 PCR product was used as positive control. The figure illustrates that the nudA gene was present only in wild type strain J99 but absent in nudA mutant strains while the CamR gene was present in nudA mutant strains but not in the wild type.